cssR

two-component response regulator (OmpR family), control of cellular responses to protein secretion stress

Locus: BSU_33010

Isoelectric point: 5.07

Molecular weight: 26.06 kDa

Protein length: 225 aa

Gene length: 678 bp

Function: control of cellular responses to protein secretion stress

Product: two-component response regulator (OmpR family)

Essential: no

Synonyms: yvqA

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Two-component system response regulators
• Lifestyles → Coping with stress → Heat shock proteins
• Groups of genes → Phosphoproteins → Phosphorylation on an Asp residue

Gene

Coordinates: 3,385,724 3,386,401

The protein

Protein family

• OmpR family of two-component response regulators

Domains

• Response regulatory domain (aa 4-117) (according to UniProt)

Modification

• phosphorylated by CssS on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Structure

• 2OQR (from Mycobacterium tuberculosis, 34% identity)

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• CssR may interact with RNA polymerase PubMed

Expression and Regulation

Operons

• Genes: cssR-cssS
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CssR: activation, PubMed, in CssR regulon

  Regulation

  • expressed under conditions of secretion stress (CssR) PubMed

Biological materials

Mutant

• MGNA-B031 (yvqA::erm), available at the NBRP B. subtilis, Japan
• BKE33010 (cssR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGGCTCTTCACATCCTTT, downstream forward: _UP4_TTCGGCTACAGGATGATGTC
• BKK33010 (cssR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGGCTCTTCACATCCTTT, downstream forward: _UP4_TTCGGCTACAGGATGATGTC

References

Reviews

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Original Publications

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