SubtiBank SubtiBank
yrrN
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yrrN

tRNA uridine hydroxylase (in complex with YrrO)
Locus
BSU_27350
Isoelectric point
4.64
Molecular weight
34.92 kDa
Protein length
309 aa Sequence Blast
Gene length
930 bp Sequence Blast
Function
introduction of 5-methoxyuridine modification in tRNA (U34)
Product
tRNA uridine hydroxylase
Essential
no
Synonyms
trhP1
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

    • Gene

    Coordinates
    2,794,147 2,795,076

    Phenotypes of a mutant

  • 50% reduction in mo5U tRNA levels PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • hydroxylation of U34 in tRNA to give 5-hydroxyuridine (ho5U) (in complex with YrrO) with prephenate as hydroxyl donor, ho5U is the precursor for 5-methoxyuridine modification (by TrmR) PubMed

    • Protein family

  • peptidase U32 family (with YrrO, according to UniProt)

    • Paralogous protein(s)

  • YrrO

    • Expression and Regulation

    Operons

    Genes
    trmR-yrrN-yrrO-udk-greA
    Description
    PubMed
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    Biological materials

    Mutant

  • MGNA-A008 (yrrN::erm), available at the NBRP B. subtilis, Japan
  • BKE27350 (yrrN::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGACGTTCACCTCTTCTT, downstream forward: _UP4_TATTAATCAAAAGGAGGTTA
  • BKK27350 (yrrN::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGACGTTCACCTCTTCTT, downstream forward: _UP4_TATTAATCAAAAGGAGGTTA

    • References

    Research papers

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