citG [Wed Aug 24 2016 13:19:20 GMT+0200 (CEST)]

fumarase

Locus: BSU33040

Isoelectric point: 5.00

Molecular weight: 50.00 kDa

Function: TCA cycle

Product: fumarate hydratase

Essential: no

E.C.: 4.2.1.2

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Carbon core metabolism → TCA cycle

Categories containing this gene/protein

• carbon core metabolism, most abundant proteins

Gene

Coordinates on the chromosome (coding sequence): 3,389,024 -> 3,390,412

The protein

Catalyzed reaction/ biological activity

• (S)-malate = fumarate H₂O (according to Swiss-Prot)

Protein family

• Fumarase subfamily (according to Swiss-Prot)

Paralogous protein(s)

• AnsB

Structure

• 3R6Q (aspartase from Bacillus sp. YM55-1, 43% identity, 77% similarity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Interactions

• Mdh-CitG PubMed
• CitG-CitB PubMed

Expression and Regulation

Operon

• citG PubMed

Sigma factor

• SigA PubMed, SigH PubMed

Regulation

• constitutive

Additional information

• belongs to the 100 most abundant proteins PubMed

Biological materials

Mutant

• GP718 (spec), available in Jörg Stülke's lab
• GP2340 (citG::kan), Cre-recombinase is integrated in sacA,available in Jörg Stülke's lab
• GP2341 (citG::lox72), Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab

Expression vector

• pGP1122 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab)

LacZ fusion

• pGP387 (in pAC7), available in Jörg Stülke's lab

GFP fusion

• GP1433 (spc, based on pGP1870), available in the Stülke lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

• GP1132 (spc, based on pGP1331), available in Jörg Stülke's lab

Antibody

• **

References

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