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citG [2019-08-09 11:35:21]
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citG [2019-08-09 11:35:21]

fumarase
Locus
BSU_33040
Isoelectric point
5.47
Molecular weight
50.36 kDa
Protein length
462 aa Sequence Blast
Gene length
1389 bp Sequence Blast
Function
TCA cycle
Product
fumarate hydratase
Essential
no
E.C.
4.2.1.2
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,389,024 3,390,412

The protein

Catalyzed reaction/ biological activity

  • (S)-malate = fumarate + H2O (according to Swiss-Prot)
  • Protein family

  • class-II fumarase/aspartase family (with AnsB, according to UniProt)
  • Paralogous protein(s)

    Structure

  • 1FUO (from E. coli, 64% identity) PubMed
  • Localization

  • forms discrete foci in the cytoplasm PubMed
  • colocalizes with DNA upon DNA damage (MMS treatment) PubMed
  • Additional information

  • belongs to the 100 most abundant proteins PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigH: sigma factor, PubMed, in SigH regulon
  • Regulation

  • constitutive
  • view in new tab

    Additional information

  • the protein amounts increase upon DNA damage (MMS treatment) PubMed
  • Biological materials

    Mutant

  • GP718 (spec), available in Jörg Stülke's lab PubMed
  • GP2340 ΔcitG::kan, Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
  • GP2341 ΔcitG::lox72, Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
  • BKE33040 (citG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTATGTATCCCTCCATA, downstream forward: _UP4_TAATAGGAAGAACGGCTGCT
  • BKK33040 (citG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTATGTATCCCTCCATA, downstream forward: _UP4_TAATAGGAAGAACGGCTGCT
  • Expression vectors

  • pGP1122 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab) PubMed
  • LacZ fusion

  • pGP387 (in pAC7), available in Jörg Stülke's lab
  • GFP fusion

  • GP1433 (spc, based on pGP1870), available in the Jörg Stülke lab
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • FLAG-tag construct

  • GP1132 (spc, based on pGP1331), available in Jörg Stülke's lab
  • Antibody

  • **
  • References

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