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citG [2019-08-09 11:35:21]
Molecular weight
50.36 kDa
Product
fumarate hydratase
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
3,389,024 3,390,412
The protein
Catalyzed reaction/ biological activity
(S)-malate = fumarate + H2O (according to Swiss-Prot)Protein family
class-II fumarase/aspartase family (with AnsB, according to UniProt) Paralogous protein(s)
Structure
Localization
forms discrete foci in the cytoplasm PubMedcolocalizes with DNA upon DNA damage (MMS treatment) PubMed Additional information
Expression and Regulation
Operons
Additional information
the protein amounts increase upon DNA damage (MMS treatment) PubMed Biological materials
Mutant
GP718 (spec), available in Jörg Stülke's lab PubMedGP2340 ΔcitG::kan, Cre-recombinase is integrated in sacA, available in Jörg Stülke's labGP2341 ΔcitG::lox72, Cre-recombinase is integrated in sacA, available in Jörg Stülke's labBKE33040 (citG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTATGTATCCCTCCATA, downstream forward: _UP4_TAATAGGAAGAACGGCTGCTBKK33040 (citG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTATGTATCCCTCCATA, downstream forward: _UP4_TAATAGGAAGAACGGCTGCT Expression vectors
LacZ fusion
GFP fusion
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab FLAG-tag construct
Antibody
**References
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