mpr [2019-06-18 09:14:12]

extracellular metalloprotease

Locus: BSU02240

Isoelectric point: 8.97

Molecular weight: 33.69 kDa

Protein length: 313 aa

Gene length: 942 bp

Function: protein degradation

Product: extracellular metalloprotease

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of proteins
• Information processing → Protein synthesis, modification and degradation → Proteolysis → Extracellular feeding proteases
• Groups of genes → Secreted proteins

Gene

Coordinates: 245,190 246,131

The protein

Protein family

• peptidase S1B family (according to Swiss-Prot)

Structure

• 1P3C (from B. intermedius, corresponds to aa 95 ... 289, 31% identity) PubMed

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: mpr-ybfJ
  Description: PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon

  Regulation

  • repressed by casamino acids PubMed

Biological materials

Mutant

• KO7 (nprE aprE epr mpr nprB vpr bpr), available as BGSC 1A1133
• BKE02240 (mpr::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTGTCATCTCCCTCCT, downstream forward: _UP4_AACTTGGGAACAAGGGTGAC
• BKK02240 (mpr::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTGTCATCTCCCTCCT, downstream forward: _UP4_AACTTGGGAACAAGGGTGAC

References

Reviews

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Original publications

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