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mpr [2019-06-18 09:14:12]
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mpr [2019-06-18 09:14:12]

extracellular metalloprotease
locus
BSU02240
pI
8.97
mw
33.69 kDa
protein length
313 aa Sequence Blast
gene length
942 bp Sequence Blast
function
protein degradation
product
extracellular metalloprotease
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.5|Utilization of proteins]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.2|Extracellular feeding proteases]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    245,190 246,131

    The protein

    Protein family

  • peptidase S1B family (according to Swiss-Prot)

    • Structure

  • [PDB|1P3C] (from B. intermedius, corresponds to aa 95 ... 289, 31% identity) [pubmed|15005613]

    • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862]

    • Expression and Regulation

    Operons

    genes
    [gene|A39D349BBE13F4F3F6D33DA72CB8149A5170255C|mpr]-[gene|22A5905A0C2A9303C166A5C2D784AF0B6541A311|ybfJ]
    description
    [Pubmed|12107147]

    regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|25666135], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

    • regulation

  • repressed by casamino acids [Pubmed|12107147]
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    Biological materials

    Mutant

  • KO7 (''nprE aprE epr mpr nprB vpr bpr''), available as BGSC 1A1133
  • BKE02240 ([gene|A39D349BBE13F4F3F6D33DA72CB8149A5170255C|mpr]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTCATCTCCCTCCT, downstream forward: _UP4_AACTTGGGAACAAGGGTGAC
  • BKK02240 ([gene|A39D349BBE13F4F3F6D33DA72CB8149A5170255C|mpr]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTCATCTCCCTCCT, downstream forward: _UP4_AACTTGGGAACAAGGGTGAC

    • References

    Reviews

  • 20735481

    • Original publications

  • 15375126,24115457,18957862,12107147,25666135,15005613