dgcW [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

diguanylate cyclase and potential phosphodiesterase

Locus: BSU13420

Isoelectric point: 6.00

Molecular weight: 84.00 kDa

Function: synthesis of c-di-GMP

Product: diguanylate cyclase and potential phosphodiesterase

Essential: no

Synonyms: ykoW

Genomic Context

Loading

Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Metabolism of signalling nucleotides
• Groups of genes → Membrane proteins

Categories containing this gene/protein

• membrane proteins, metabolism of signalling nucleotides

This gene is a member of the following regulons

• SigD regulon

Gene

Coordinates on the chromosome (coding sequence): 1,407,329 -> 1,409,578

The protein

Catalyzed reaction/ biological activity

• synthesis of c-di-GMP from two molecules of GTP PubMed

Domains

• contains a N-terminal PAS domain PubMed
• contains a central GGDEF domain and a C-terminal EAL domain PubMed
• aa 290-735 are similar to E. coli CsrD (18% identity, 43% similarity)

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operon

• dgcW PubMed

Sigma factor

• SigD PubMed

Biological materials

Mutant

• GP850 (ykoW::cat), available in Jörg Stülke's lab
• BP140 (ykoW::ermC) available in Fabian Commichau's lab
• BP142 (ykoW-ykoV-ligD::kan) available in Fabian Commichau's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

Loading