dgcW [Fri Dec 09 2016 17:14:05 GMT+0100 (CET)]

diguanylate cyclase and potential phosphodiesterase

locus: BSU13420

pI: 6.00

mw: 84.00 kDa

function: synthesis of c-di-GMP

product: diguanylate cyclase and potential phosphodiesterase

essential: no

synonyms: ykoW

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

[SW|Categories] containing this gene/protein

• [SW|membrane proteins], [SW|metabolism of signalling nucleotides]

This gene is a member of the following [SW|regulons]

• [SW|SigD regulon]

Gene

Coordinates on the chromosome (coding sequence): 1,407,329 -> 1,409,578

The protein

Catalyzed reaction/ biological activity

• synthesis of c-di-GMP from two molecules of GTP [Pubmed|23893111]

[SW|Domains]

• contains a N-terminal [SW|PAS domain] [Pubmed|23893111]
• contains a central [SW|GGDEF domain] and a C-terminal [SW|EAL domain] [Pubmed|22821967]
• aa 290-735 are similar to ''E. coli'' CsrD (18% identity, 43% similarity)

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operon

• ''[protein|search|dgcW]'' [Pubmed|22383849]

[SW|Sigma factor]

• [SW|SigD] [Pubmed|26577401]

Biological materials

Mutant

• GP850 (''[protein|search|dgcW]''::''cat''), available in [SW|Jörg Stülke]'s lab
• BP140 (''[protein|search|dgcW]''::''ermC'') available in [SW|Fabian Commichau]'s lab
• BP142 (''[protein|search|dgcW]-[protein|search|ykoV]-[protein|search|ligD]''::''kan'') available in [SW|Fabian Commichau]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

References

• 11728710,16980588,22821967,22383849,23893111,26577401