yhbF

bactofilin, required for flagellar hook- and filament assembly

locus: BSU_08960

pI: 6.64

mw: 24.95 kDa

protein length: 235 aa

gene length: 708 bp

function: assembly of the flagellar hook and filament

product: bactofilin

essential: no

synonyms: yzdB

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.2|Flagellar proteins]

Gene

Coordinates: 972,099 972,806

Phenotypes of a mutant

• complete loss of swimming motility [Pubmed|26517549]

The protein

Paralogous protein(s)

• [protein|CC0B2F230BD9F0F05B1DAF5BB2C92A9E30CDB5B7|YhbE]

[SW|Localization]

• discrete assemblies at the cell membrane [Pubmed|26517549]
• assemblies are relatively static, and partially colocalize with flagellar basal bodies [Pubmed|26517549]

Expression and Regulation

Operons

• genes: [gene|FDA5363A4DB711CD799BEFF395D58072CD8FFC44|yhbD]-[gene|CC0B2F230BD9F0F05B1DAF5BB2C92A9E30CDB5B7|yhbE]-[gene|A283DF56E47D601004F660809AB7BD32C644D422|yhbF]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A733 (yhbF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/733 NBRP B. subtilis, Japan]
• BKE08960 ([gene|A283DF56E47D601004F660809AB7BD32C644D422|yhbF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTTGTCTCCATTACGATCC, downstream forward: _UP4_TAAAACATTGTTCTGTTATA
• BKK08960 ([gene|A283DF56E47D601004F660809AB7BD32C644D422|yhbF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTTGTCTCCATTACGATCC, downstream forward: _UP4_TAAAACATTGTTCTGTTATA

References

• 8626065,22383849,26517549