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crh [2018-02-13 09:34:59]
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crh [2018-02-13 09:34:59]

"Carbon-flux regulating HPr", formerly known as "Catabolite repression HPr-like protein", control of flux through the harmful methylglyoxal pathway, minor cofactor of the CcpA transcription factor
locus
BSU34740
pI
4.70
mw
9.19 kDa
protein length
85 aa Sequence Blast
gene length
255 bp Sequence Blast
function
control of carbon flux
product
carbon-flux regulating HPr
essential
no
synonyms
yvcM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • Gene

    Coordinates
    3,569,292 → 3,569,549

    The protein

    Protein family

  • [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr]]] family

    • Paralogous protein(s)

  • [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr]]]

    • [SW|Domains]

  • [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr]]] domain (1–85)

    • Modification

  • phosphorylation on Ser46 by [protein|771F205F818A755A661B8E1C95365A4F6AEB05C7|HprK] [Pubmed|9237995,21992469,22092971]

    • Structure

  • [PDB|2AK7] (dimeric Crh-Ser46-P)
  • [PDB|1ZVV] ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]-Crh-DNA complex)
  • [PDB|2RLZ] (dimer)
  • [PDB|1MU4]
  • [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=24634 NCBI], dimer
  • [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=64943 NCBI], [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]-Crh-DNA complex
  • [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=52326 NCBI], dimeric phosphor-Crh
  • [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=39567 NCBI]

    • Additional information

  • Crh does not possess the phosphorylation site used for [protein|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|PtsI] phosphotransfer (His-15 in [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|PtsH]), it can only be phosphorylated on Ser-46

    • Expression and Regulation

    Operons

    genes
    [gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]-[gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]-[gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR]-[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]-[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]-[gene|C521BEE1EEEA7AD283A3C47C34301A8603CD9188|yvcN]
    description
    [Pubmed|9237995]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16272399], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • constitutive expression at both protein and RNA levels [Pubmed|24097947,22383849]

    • additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|yvcI]' and '[protein|search|trxB]' [PubMed|20525796]
    • view in new tab

    Biological materials

    Mutant

  • GP860 (aphA3) [Pubmed|21992469], QB7097 (spc), available in [SW|Jörg Stülke]'s lab
  • BKE34740 (Δ[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGATCTCCCCTTTTCT, downstream forward: _UP4_GCTTACGTTCAAGAAGAAGT
  • BKK34740 (Δ[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGATCTCCCCTTTTCT, downstream forward: _UP4_GCTTACGTTCAAGAAGAAGT

    • Expression vector

  • pGP641 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
  • pGP734 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • see ''[gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]

    • two-hybrid system

  • ''[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]'', ''crh(Ser46Asp)'', ''crh(Ser46Ala)'' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Boris Görke], University of Göttingen, Germany
  • [http://wwwuser.gwdg.de/~genmibio/goerke.html Homepage]
  • [SW|Anne Galinier], University of Marseille, France
  • [SW|Wolfgang Hillen], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]
  • [SW|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]

    • References

  • 12972249,9973552,9237995,16272399,15126459,10217795,17142398,16316990,12009882,10589728,12670692,11916384,11361076,11361074,18320329,18284240,16411239,21992469,22092971