ykuJ

unknown

locus: BSU_14100

pI: 4.34

mw: 9.11 kDa

protein length: 79 aa

gene length: 240 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 1,484,117 1,484,356

The protein

Structure

• [PDB|2FFG]

Expression and Regulation

Operons

• genes: [gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]-[gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]-[gene|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA]-[gene|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]-[gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]
  description: [Pubmed|16395550]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, by [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]~P [Pubmed|18840696,14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|16395550,12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

Biological materials

Mutant

• MGNA-A771 (ykuJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/771 NBRP B. subtilis, Japan]
• GP214 (deletion of ''[gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]-[gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]-[gene|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA]-[gene|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]'', replaced by ''aphA3''), available in [SW|Jörg Stülke]'s lab
• BKE14100 ([gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTAAAAAAAGACTCCT, downstream forward: _UP4_TAAGATTCCTGAAATAGGGG
• BKK14100 ([gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTAAAAAAAGACTCCT, downstream forward: _UP4_TAAGATTCCTGAAATAGGGG

lacZ fusion

• pGP710 (in [SW|pAC5]), available in [SW|Jörg Stülke]'s lab, pGP714 (in [SW|pAC6]), available in [SW|Jörg Stülke]'s lab

References

• 16395550