ywcH [2017-05-10 18:48:56]

similar to monooxygenase

locus: BSU38100

pI: 5.94

mw: 36.40 kDa

protein length: 333 aa

gene length: 999 bp

function: unknown

product: unknown

essential: no

synonyms: ipa-44d

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 3,910,302 → 3,911,303

The protein

Protein family

• Flavin-utilizing monoxygenases

Paralogous protein(s)

• [protein|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|CmoO], [protein|1432F56AD3D2B2BD82D8759CE4DC42C0D40C49C0|YceB], [protein|A36E7D50E2EBD0F0109491FF2CAD2CA84372B2CF|YvbT]

Structure

• [PDB|4US5] (39% identity)

Expression and Regulation

Operons

• genes: [gene|5B95F7CFEFCB954FCBC11087F030A1D777F34B7A|nfrA]-[gene|A1DCFF8D225C87197104742E72A0D4F238BC30EB|ywcH]
  description: [Pubmed|10913069]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10913069], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|9535080], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|29271514,12642660,19575568], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

  regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
  • induced by cell wall stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]) [pubmed|29271514]

Biological materials

Mutant

• MGNA-B233 (ywcH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1232 NBRP B. subtilis, Japan]

References

• 10913069,9535080,14651647