SubtiBank SubtiBank
rph
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

rph

RNase PH, 3-5 exoribonuclease
Locus
BSU_28370
Isoelectric point
4.89
Molecular weight
26.53 kDa
Protein length
245 aa Sequence Blast
Gene length
738 bp Sequence Blast
Function
3-5 exoribonuclease
Product
RNase PH (EC 2.7.7.56)
Essential
no
E.C.
2.7.7.56
Synonyms

Genomic Context

      
Loading

Categories containing this gene/protein

Gene

Coordinates
2,901,154 2,901,891

The protein

Catalyzed reaction/ biological activity

  • major player in the exonucleolytic maturation of CCA-containing tRNA precursors PubMed
  • responsible for endonucleolytic cleavage of scRNA (scr) PubMed
  • phosphate + tRNA(n+1) --> ribonucleoside 5'-diphosphate + tRNA(n) (according to UniProt)
  • Protein family

  • RNase PH family (single member, according to UniProt)
  • Structure

    Expression and Regulation

    Operons

    Biological materials

    Mutant

  • BKE28370 (rph::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTTACCTCCGATTGT, downstream forward: _UP4_TAAAGACAGAAAGGCTTGAT
  • BKK28370 (rph::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTTACCTCCGATTGT, downstream forward: _UP4_TAAAGACAGAAAGGCTTGAT
  • Labs working on this gene/protein

  • David Bechhofer, Mount Sinai School, New York, USA Homepage
  • Ciaran Condon, IBPC, Paris, France Homepage
  • References

    Reviews

    Loading

    Original Publications

    Loading