rph

RNase PH, 3-5 exoribonuclease

Locus: BSU_28370

Isoelectric point: 4.89

Molecular weight: 26.53 kDa

Protein length: 245 aa

Gene length: 738 bp

Function: 3-5 exoribonuclease

Product: RNase PH (EC 2.7.7.56)

Essential: no

E.C.: 2.7.7.56

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → RNases → Exoribonucleases
• Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

Gene

Coordinates: 2,901,154 2,901,891

The protein

Catalyzed reaction/ biological activity

• major player in the exonucleolytic maturation of CCA-containing tRNA precursors PubMed
• responsible for endonucleolytic cleavage of scRNA (scr) PubMed
• phosphate + tRNA_(n+1) --> ribonucleoside 5'-diphosphate + tRNA_(n) (according to UniProt)

Protein family

• RNase PH family (single member, according to UniProt)

Structure

• 1OYS

Expression and Regulation

Operons

• Genes: rph-ysnA-ysnB
  Description: PubMed

Biological materials

Mutant

• BKE28370 (rph::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTTACCTCCGATTGT, downstream forward: _UP4_TAAAGACAGAAAGGCTTGAT
• BKK28370 (rph::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTTACCTCCGATTGT, downstream forward: _UP4_TAAAGACAGAAAGGCTTGAT

Labs working on this gene/protein

• David Bechhofer, Mount Sinai School, New York, USA Homepage
• Ciaran Condon, IBPC, Paris, France Homepage

References

Reviews

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Original Publications

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