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yrbD [2020-07-10 11:10:48]
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yrbD [2020-07-10 11:10:48]

sodium/proton-dependent alanine transporter
locus
BSU_27810
pI
9.38
mw
51.43 kDa
protein length
484 aa Sequence Blast
gene length
1455 bp Sequence Blast
function
uptake of alanine
product
sodium/proton-dependent alanine transporter
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.3|Alanine or glycine cation symporter family]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.8|Biosynthesis/ acquisition of serine/ glycine/ alanine]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.7|Utilization of alanine/ serine]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]

    • Gene

    Coordinates
    2,841,611 2,843,065

    The protein

    Protein family

  • [SW|Alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|YflA], [protein|B84BE9F5BEFE3A295ED5580D66040CD28609A6A1|AlsT], [protein|E371BC4F613C40CB0E8C8CAA97387F3E6735D813|GlnT]

    • Structure

  • [PDB|6CSE] (sodium/alanine symporter from Methanococcus maripaludis S2, 37% identity) [pubmed|30659158]

    • Expression and Regulation

    Operons

    genes
    [gene|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|yrbD]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
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    Biological materials

    Mutant

  • MGNA-A821 (yrbD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/821 NBRP B. subtilis, Japan]
  • BKE27810 ([gene|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|yrbD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGCTTCCCCCTCACT, downstream forward: _UP4_TGAACATACTAAAACCGGCC
  • BKK27810 ([gene|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|yrbD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGCTTCCCCCTCACT, downstream forward: _UP4_TGAACATACTAAAACCGGCC

    • References

  • 25755103,30659158