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zur [2018-12-18 18:19:41]
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zur [2018-12-18 18:19:41]

transcriptional repressor, regulates zinc homeostasis
locus
BSU25100
pI
4.86
mw
16.42 kDa
protein length
145 aa Sequence Blast
gene length
435 bp Sequence Blast
function
regulation of zinc homeostasis([gene|75DB07A2704FEC2932BD0CDD24E2B3454E9551E1|zagA], [gene|D33A144568CAF32FFE4A2A46BE67573DB66FC1A1|znuA]-[gene|6B5D4FD32CCE72C99B915B0F1EB4B8E8D42A04B3|znuC]-[gene|EE212953BC83BD6D69BF769D6E917F4F954BE1E2|znuB])
product
transcriptional repressor ([SW|Fur family])
essential
no
synonyms
yqfV
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.3|Zinc]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

    • Gene

    Coordinates
    2,591,428 → 2,591,865

    The protein

    Protein family

  • [SW|Fur family]

    • Paralogous protein(s)

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur], [protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR]

    • Modification

  • phosphorylated on ser/ thr/ tyr [Pubmed|16493705]

    • Effectors of protein activity

  • sequential binding of zinc (two atoms per monomer) results in the activation of Zur dimer and thus in repression of the genes of the [SW|Zur regulon] [Pubmed|21821657]
  • zinc binding is negatively co-operative resulting in step-wise induction of the genes of the [SW|Zur regulon] upon zinc depletion [Pubmed|27561249]

    • Structure

  • [PDB|2FE3] ([protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR], 35% identity)

    • [SW|Localization]

  • information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000090 PRODORIC2 database]

    • Expression and Regulation

    Operons

    genes
    [gene|F2B25938E665BD4FB06DD3A09EDA0FA1AB101B24|yqfU]-[gene|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • 1A904 ( ''zur''::''spec''), [Pubmed|12029044], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A904&Search=1A904 BGSC]
  • BKE25100 (Δ[gene|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAGGGTCCCCCTTTTC, downstream forward: _UP4_TAAAATGCGTATATATGAAA
  • BKK25100 (Δ[gene|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAGGGTCCCCCTTTTC, downstream forward: _UP4_TAAAATGCGTATATATGAAA

    • labs

  • [SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]

    • References

  • 21821657,18344368,14563870,12904577,16493705,9811636,12426338,19648245,25649915,27561249