SubtiBank SubtiBank
uxuA
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uxuA

D-mannonate hydrolyase
Locus
BSU_12340
Isoelectric point
4.92
Molecular weight
40.87 kDa
Protein length
359 aa Sequence Blast
Gene length
1080 bp Sequence Blast
Function
hexuronate utilization
Product
D-mannonate hydrolyase
Essential
no
E.C.
4.2.1.8
Synonyms
yjmE

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,305,486 1,306,565

The protein

Catalyzed reaction/ biological activity

  • D-mannonate --> 2-dehydro-3-deoxy-D-gluconate + H2O (according to UniProt)
  • Protein family

  • mannonate dehydratase family (single member, according to UniProt)
  • Structure

  • 1TZ9 (from Enterococcus faecalis, 44% identity)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigE: sigma factor, PubMed, in SigE regulon
  • Regulatory mechanism

  • ExuR: repression, in the absence of inducer, in ExuR regulon
  • CcpA: repression, PubMed PubMed, in CcpA regulon
  • Regulation

  • induced by galacturonate (ExuR) PubMed
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    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • ExuR: repression, in the absence of inducer, in ExuR regulon
  • CcpA: repression, PubMed PubMed, in CcpA regulon
  • Regulation

  • induced by galacturonate (ExuR) PubMed
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    Biological materials

    Mutant

  • MGNA-A369 (yjmE::erm), available at the NBRP B. subtilis, Japan
  • BKE12340 (uxuA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTCGCACCTCCGGATT, downstream forward: _UP4_ATGGCAAAAAAAGAGGTGGG
  • BKK12340 (uxuA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTCGCACCTCCGGATT, downstream forward: _UP4_ATGGCAAAAAAAGAGGTGGG
  • References

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