SubtiBank SubtiBank
ktrD [2019-02-14 12:07:28]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

ktrD [2019-02-14 12:07:28]

low affinity potassium channel [protein|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|KtrC]-[protein|A0952B75E5BD0D09B0F4C257822428640998558B|KtrD], integral membrane subunit
locus
BSU13500
pI
9.22
mw
49.26 kDa
protein length
449 aa Sequence Blast
gene length
1350 bp Sequence Blast
function
potassium uptake
product
low affinity potassium channel [protein|15F9BF08762D28CBCD3D9748EFBD29B0D6FD7623|KtrC]-[protein|A0952B75E5BD0D09B0F4C257822428640998558B|KtrD], integral membrane subunit
essential
no
synonyms
ykrM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,416,067 1,417,416

    The protein

    Catalyzed reaction/ biological activity

  • uptake of potassium

    • Protein family

  • View classification (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB]

    • Structure

  • [PDB|4J7C] (the [protein|92D0275E0768780F782F4B3724BC5181E436B6B1|KtrA]-[protein|A287B9771A56D03ADCA603C1969CBF03DCB10E95|KtrB] complex, 36% identity) [Pubmed|23598340]

    • [SW|Localization]

  • integral membrane protein [Pubmed|12562800]

    • Expression and Regulation

    Operons

    genes
    [gene|A0952B75E5BD0D09B0F4C257822428640998558B|ktrD]
    description
    [Pubmed|12562800]

    regulation

  • constitutively expressed
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B317 (ykrM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1316 NBRP B. subtilis, Japan]
  • GHB12 (''ktrD''::''tet''), [Pubmed|12562800], available in [SW|Erhard Bremer]'s and [SW|Jrg Stlke]'s labs
  • GP2030 (''ktrD''::''tet''), available in [SW|Jrg Stlke]'s lab [pubmed|28420751]
  • 1A956 (''ktrD''::''tet''), [Pubmed|12562800], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A956&Search=1A956 BGSC]
  • BKE13500 ([gene|A0952B75E5BD0D09B0F4C257822428640998558B|ktrD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAATCCTTCTGCCT, downstream forward: _UP4_TAGGCAAAACACCGCATATT
  • BKK13500 ([gene|A0952B75E5BD0D09B0F4C257822428640998558B|ktrD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAATCCTTCTGCCT, downstream forward: _UP4_TAGGCAAAACACCGCATATT

    • FLAG-tag construct

  • GP2434 ''ktrD-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jrg Stlke]'s lab

    • labs

  • [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

    • References

    Reviews

  • 21680052,27935846,25838295

    • Original publications

  • 12562800,23598340,28420751,28504641