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bkdAB [2018-12-09 18:11:31]
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bkdAB [2018-12-09 18:11:31]

2-oxoisovalerate dehydrogenase (E1 beta subunit)
locus
BSU24040
pI
4.79
mw
35.70 kDa
protein length
327 aa Sequence Blast
gene length
981 bp Sequence Blast
function
utilization of branched-chain keto acids
product
2-oxoisovalerate dehydrogenase (E1 beta subunit)
essential
no
ec
1.2.4.4
synonyms
bfmBAB, bfmB1b, bkd
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • Gene

    Coordinates
    2,498,093 → 2,499,076

    The protein

    Catalyzed reaction/ biological activity

  • 3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2 (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|458E967052D1093A0F48AE0E6B6CCA0F52EAC44D|PdhB], [protein|5F953C3C91EB65A031D04A1D91F1CF501F8EFD09|AcoB]

    • [SW|Localization]

  • Cytoplasm (Homogeneous) [Pubmed|16479537]

    • Expression and Regulation

    Operons

    genes
    [gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]-[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd]-[gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]-[gene|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV]-[gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]-[gene|A024921961A786294199EA12E04456C890ED8D8C|bkdAB]-[gene|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB]
    description
    [Pubmed|12823818]

    sigma factors

  • [protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL]: sigma factor, [Pubmed|10094682], in [regulon|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL regulon]

    • regulatory mechanism

  • [protein|4CEBD81F485DD0B660E297FFC34A0F5270652184|BkdR]: activation, [Pubmed|10094682], in [regulon|4CEBD81F485DD0B660E297FFC34A0F5270652184|BkdR regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|10094682], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

    • regulation

  • induced in the presence of isoleucine or valine ([protein|search|BkdR]) [Pubmed|10094682]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C437 (bfmBAB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2435 NBRP B. subtilis, Japan]
  • BKE24040 (Δ[gene|A024921961A786294199EA12E04456C890ED8D8C|bkdAB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACTGACATTTGTTCTT, downstream forward: _UP4_TAAAGACGTAAGGGAGGATA
  • BKK24040 (Δ[gene|A024921961A786294199EA12E04456C890ED8D8C|bkdAB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACTGACATTTGTTCTT, downstream forward: _UP4_TAAAGACGTAAGGGAGGATA

    • References

  • 12427936,15241682,10094682,12823818,16479537,1886522