bkdAB [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

2-oxoisovalerate dehydrogenase (E1 beta subunit)

locus: BSU24040

pI: 4.79

mw: 35.00 kDa

function: utilization of branched-chain keto acids

product: 2-oxoisovalerate dehydrogenase (E1 beta subunit)

essential: no

ec: 1.2.4.4

synonyms: bfmBAB, bfmB1b, bkd

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

[SW|Categories] containing this gene/protein

• [SW|utilization of amino acids]

This gene is a member of the following [SW|regulons]

• [SW|BkdR regulon], [SW|CodY regulon], [SW|SigL regulon]

Gene

Coordinates on the chromosome (coding sequence): 2,498,093 -> 2,499,076

The protein

Catalyzed reaction/ biological activity

• 3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO₂ (according to Swiss-Prot)

Paralogous protein(s)

• [protein|search|PdhB], [protein|search|AcoB]

[SW|Localization]

• Cytoplasm (Homogeneous) [Pubmed|16479537]

Expression and Regulation

Operon

• ''[protein|search|ptb]-[protein|search|bcd]-[protein|search|buk]-[protein|search|lpdV]-[SW|bkdAA]-[SW|bkdAB]-[protein|search|bkdB]'' [Pubmed|12823818]

[SW|Sigma factor]

• [protein|search|SigL] [Pubmed|10094682]

Regulation

• induced in the presence of isoleucine or valine ([protein|search|BkdR]) [Pubmed|10094682]

Regulatory mechanism

• [protein|search|CodY]: transcription repression [Pubmed|10094682]
• [protein|search|BkdR]: transcription activation [Pubmed|10094682]

References

• 12427936,15241682,10094682,12823818,16479537,1886522