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ynbA [2019-09-16 12:38:51]
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ynbA [2019-09-16 12:38:51]

GTP-binding protein, ribosome-splitting factor, rescues translationally arrested ribosomes
Locus
BSU_17430
Isoelectric point
9.24
Molecular weight
37.51 kDa
Protein length
420 aa Sequence Blast
Gene length
1263 bp Sequence Blast
Function
rescuing of translationally arrested ribosomes
Product
GTP-binding protein, ribosome-splitting factor
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,875,304 1,876,566

The protein

Catalyzed reaction/ biological activity

  • binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • rescuing of translationally arrested ribosomes PubMed
  • Protein family

  • TRAFAC class OBG-HflX-like GTPase superfamily (with Obg and YyaF, according to UniProt)
  • Paralogous protein(s)

    Effectors of protein activity

  • binds ppGpp PubMed, ppGpp may inhibit activity PubMed
  • Structure

  • 5ADY (the protein from E. coli in complex with the ribosome, 41% identity) PubMed
  • Expression and Regulation

    Operons

    Description

    Regulation

  • the mRNA is processed between glnR and glnA by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed
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    Biological materials

    Mutant

  • MGNA-A020 (ynbA::erm), available at the NBRP B. subtilis, Japan
  • BKE17430 (ynbA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAATGGATGTTCCTTTCTT, downstream forward: _UP4_ATGTAGGAAGGAAATATAAA
  • BKK17430 (ynbA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAATGGATGTTCCTTTCTT, downstream forward: _UP4_ATGTAGGAAGGAAATATAAA
  • Labs working on this gene/protein

  • Naotake Ogasawara, Nara, Japan
  • References

    Reviews

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    Original publications

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