bkdAA [Mon Aug 31 2015 13:55:29 GMT+0200 (CEST)]

2-oxoisovalerate dehydrogenase (E1 alpha subunit)

locus: BSU24050

pI: 4.78

mw: 36.00 kDa

function: utilization of branched-chain keto acids

product: 2-oxoisovalerate dehydrogenase (E1 alpha subunit)

essential: no

ec: 1.2.4.4

synonyms: bfmBAA, bfmB1a, bkd

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]

syns: bfmBAA, bfmB1a, bkd

[SW|Categories] containing this gene/protein

• [SW|utilization of amino acids]

This gene is a member of the following [SW|regulons]

• [SW|BkdR regulon], [SW|CodY regulon], [SW|SigL regulon]

The protein

Catalyzed reaction/ biological activity

• 3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO₂ (according to Swiss-Prot)

Protein family

• BCKDHA family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|search|AcoA], [protein|search|PdhA]

Structure

• [PDB|1UMB] (from '' Thermus thermophilus'', 42% identity, 57% similarity) [Pubmed|15033367]

[SW|Localization]

• Membrane-proximal (Spotty) [Pubmed|16479537]

Expression and Regulation

Operon

• ''[protein|search|ptb]-[protein|search|bcd]-[protein|search|buk]-[protein|search|lpdV]-[SW|bkdAA]-[SW|bkdAB]-[protein|search|bkdB]'' [Pubmed|12823818]

[SW|Sigma factor]

• [protein|search|SigL] [Pubmed|10094682]

Regulation

• induced in the presence of isoleucine or valine ([protein|search|BkdR]) [Pubmed|10094682]

Regulatory mechanism

• [protein|search|CodY]: transcription repression [Pubmed|10094682]
• [protein|search|BkdR]: transcription activation [Pubmed|10094682]

References

• 12427936,15241682,10094682,12823818,16479537,1886522