ydiO

similar to DNA-3-methyladenine glycosidase II

locus: BSU_06060

pI: 7.66

mw: 48.75 kDa

protein length: 427 aa

gene length: 1284 bp

function: BsuM DNA modification

product: unknown

essential: yes

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.4|DNA restriction/ modification]
• [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.5|Prophage 3]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 655,223 656,506

Phenotypes of a mutant

• essential [Pubmed|12682299], mutation is only possible in a ''[gene|4BD4CFB10700907DF1BBF3C9CFACAF573588E85F|ydiR]'', ''[gene|A8C606348C8C6FC3BB9786C06C9BFE9D3519A041|ydiS]'', or ''[gene|D36B91B3C8EAFEB80A7395C62A50A5F80B8CC5C5|ydjA]'' background (''i. e.'', in the absence of the BsuM DNA restriction system) [Pubmed|11751814]

The protein

Catalyzed reaction/ biological activity

• 2'-deoxycytidine in DNA + S-adenosyl-L-methionine --> 5-methyl-2'-deoxycytidine in DNA + H⁺ + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• [SW|Methyltransferase superfamily] (according to UniProt)
• [SW|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)

[SW|Domains]

• SAM-dependent MTase C5-type domain (aa 84-427) (according to UniProt)

Structure

• [PDB|3G7U] (from E. coli phage, 28% identity)

Expression and Regulation

Operons

• genes: [gene|9EE25F97B2C6240E31BA1D5EEE9DA2D7940C9637|ydiO]-[gene|CEA337DD4C7DFC22247FDA7FA1E71356FB819C77|ydiP]
  description: [Pubmed|11751814]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

  regulation

  • induced by DNA damage ([protein|search|LexA]) [Pubmed|16267290]

References

• 16267290,11751814,22092861