dppA

D-alanyl-aminopeptidase

locus: BSU_12920

pI: 5.19

mw: 30.09 kDa

protein length: 274 aa

gene length: 825 bp

function: degradation of cell wall peptides

product: D-alanyl-aminopeptidase

essential: no

synonyms: dciAA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.4|Utilization of peptides]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

Gene

Coordinates: 1,360,401 1,361,225

The protein

Protein family

• peptidase M55 family (single member, according to UniProt)

Modification

• phosphorylated on ser/ thr/ tyr [Pubmed|16493705]

Structure

• [PDB|1HI9]

Expression and Regulation

Operons

• genes: [gene|9E922F0006DF9E84A8D5B51E9FC35B669872FF90|dppA]-[gene|062AE5FB4C778178A7F6833CB8BD9D631E461E89|dppB]-[gene|EDF8D2C49FA5552B24135E12524F9035E1E01473|dppC]-[gene|7FA72D949B930A3C7FF0391CC6CCC58EEFCA839A|dppD]-[gene|853C570CFB9382449A20BE77B44B4FE44972AA59|dppE]
  description: [Pubmed|1766371]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1766371], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|7783641], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

  regulation

  • repressed by glucose (2.9-fold) [Pubmed|12850135]

• genes: [gene|9E922F0006DF9E84A8D5B51E9FC35B669872FF90|dppA]-[gene|062AE5FB4C778178A7F6833CB8BD9D631E461E89|dppB]-[gene|EDF8D2C49FA5552B24135E12524F9035E1E01473|dppC]-[gene|7FA72D949B930A3C7FF0391CC6CCC58EEFCA839A|dppD]-[gene|853C570CFB9382449A20BE77B44B4FE44972AA59|dppE]-[gene|C4F85F85F0C99A59145961E34B19D3AD03D2BC22|ykfA]-[gene|77EFC47449D8CC17651ED61C41AC605FCEF01270|ykfB]-[gene|D40ECAE51D9E4791C6FAAA25E2E0D0591D9FAA55|ykfC]-[gene|BB94F5388A0D1FF025E5A88A7E10AE7E4C489E5F|ykfD]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12618455], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

Biological materials

Mutant

• BKE12920 ([gene|9E922F0006DF9E84A8D5B51E9FC35B669872FF90|dppA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACGCTCCTCCTTTTTT, downstream forward: _UP4_TTCTGCTAAAGGGGTGTTTT
• BKK12920 ([gene|9E922F0006DF9E84A8D5B51E9FC35B669872FF90|dppA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACGCTCCTCCTTTTTT, downstream forward: _UP4_TTCTGCTAAAGGGGTGTTTT

References

• 18083814,12618455,1766371,19602397,7783641,1766371,12850135,16493705