hypO

NADPH-flavin nitroreductase

locus: BSU_07830

pI: 5.67

mw: 25.48 kDa

protein length: 221 aa

gene length: 666 bp

function: protection against NaOCl stress

product: NADPH-flavin nitroreductase

essential: no

synonyms: yfkO

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.4|Electron transport/ other/ based on similarity]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 854,412 855,077

Phenotypes of a mutant

• a ''[gene|9DF5999568760E016ECA988385A1FA61A257D486|hypO] [gene|869E46AECF6249610F8541E403D834284B62171D|ohrA]'' mutant is more sensitive to NaOCl stress than ''[gene|869E46AECF6249610F8541E403D834284B62171D|ohrA]'' single mutant [Pubmed|22238377]

The protein

Catalyzed reaction/ biological activity

• reduction of 2,6-dinitrotoluene [pubmed|31392514]

Protein family

• [SW|nitroreductase family] (according to UniProt)

[SW|Cofactors]

• FMN [Pubmed|21635694] (according to UniProt)
• NADP [pubmed|31392514]

Structure

• [PDB|2H0U] (from Helicobacter pylori, 45% identity)

Expression and Regulation

Operons

• genes: [gene|9DF5999568760E016ECA988385A1FA61A257D486|hypO]
  description: [Pubmed|22238377]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22238377], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|91085DA5E71B88E533D2E1D1BFB53E908116C6AA|HypR]: activation, [Pubmed|22238377], in [regulon|91085DA5E71B88E533D2E1D1BFB53E908116C6AA|HypR regulon]

  regulation

  • induced under disulfide stress conditions (diamide, NaOCl) ([protein|search|HypR]) [Pubmed|22238377]

Biological materials

Mutant

• MGNA-C366 (yfkO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2364 NBRP B. subtilis, Japan]
• BKE07830 ([gene|9DF5999568760E016ECA988385A1FA61A257D486|hypO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAACACCTCTCCTT, downstream forward: _UP4_TAAAAGAAGAAAGCTGCTCG
• BKK07830 ([gene|9DF5999568760E016ECA988385A1FA61A257D486|hypO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAACACCTCTCCTT, downstream forward: _UP4_TAAAAGAAGAAAGCTGCTCG

labs

• [SW|Haike Antelmann], University of Greifswald, Germany

References

Original publications

• 20727918,31392514,32851465