ganB [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

endo-1,4-beta-galactosidase

locus: BSU34120

pI: 8.24

mw: 47.00 kDa

function: galactan utilization

product: endo-1,4-beta-galactosidase

essential: no

ec: 3.2.1.89

synonyms: yvfO

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.24|Utilization of galactan]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

[SW|Categories] containing this gene/protein

• [SW|utilization of specific carbon sources]

This gene is a member of the following [SW|regulons]

• [SW|LacR regulon]

Gene

Coordinates on the chromosome (coding sequence): 3,501,651 -> 3,502,940

Phenotypes of a mutant

• a ''[protein|search|cycB]-[protein|search|ganP]-[protein|search|ganQ]-[protein|search|ganA]-[protein|search|ganB]'' mutant is impaired in the utilization of galactan [Pubmed|22893383]

The protein

Catalyzed reaction/ biological activity

• Endohydrolysis of (1->4)-beta-D-galactosidic linkages in galactan, generation of galactotriose [Pubmed|22893383]

Protein family

• glycosyl hydrolase 53 family (according to Swiss-Prot)

Structure

• [PDB|2J74] (B. licheniformis)

[SW|Localization]

• likely to be extrecellular [Pubmed|22893383]

Expression and Regulation

Operon

• ''[protein|search|cycB]-[protein|search|ganP]-[protein|search|ganQ]-[protein|search|ganA]-[protein|search|ganB]'' [Pubmed|9287030]

Regulatory mechanism

• [protein|search|LacR]: transcription repression [Pubmed|9287030]

References

• 17056685,9287030,22893383