ganB

endo-1,4-beta-galactosidase

Locus: BSU_34120

Isoelectric point: 8.24

Molecular weight: 47.12 kDa

Protein length: 429 aa

Gene length: 1290 bp

Function: galactan utilization

Product: endo-1,4-beta-galactosidase

Essential: no

E.C.: 3.2.1.89

Synonyms: yvfO

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of galactan
• Groups of genes → Secreted proteins

Gene

Coordinates: 3,501,651 3,502,940

Phenotypes of a mutant

• a ganS-ganP-ganQ-ganA-ganB mutant is impaired in the utilization of galactan PubMed

The protein

Catalyzed reaction/ biological activity

• Endohydrolysis of (14)-beta-D-galactosidic linkages in galactan, generation of galactotriose PubMed

Protein family

• glycosyl hydrolase 53 family (single member, according to UniProt)

Structure

• 2J74 (B. licheniformis)

Localization

• likely to be extracellular, carries a predicted a signal peptide of 26 amino acid residues at the N-terminus PubMed

Expression and Regulation

Operons

• Genes: ganS-ganP-ganQ-ganA-ganB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • GanR: repression, PubMed, in GanR regulon
  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • induced by galactan (binding of galactobiose to GanR) PubMed
  • repressed by glucose (CcpA) PubMed

Biological materials

Mutant

• MGNA-A487 (yvfO::erm), available at the NBRP B. subtilis, Japan
• BKE34120 (ganB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATTTCATCCCCCCAAG, downstream forward: _UP4_TGAAACAAAAAAATCCATGA
• BKK34120 (ganB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATTTCATCCCCCCAAG, downstream forward: _UP4_TGAAACAAAAAAATCCATGA

References

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