ganB

endo-1,4-beta-galactosidase

locus: BSU_34120

pI: 8.24

mw: 47.12 kDa

protein length: 429 aa

gene length: 1290 bp

function: galactan utilization

product: endo-1,4-beta-galactosidase

essential: no

ec: 3.2.1.89

synonyms: yvfO

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.24|Utilization of galactan]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 3,501,651 3,502,940

Phenotypes of a mutant

• a ''[gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]-[gene|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]-[gene|676932E7DF143DC9AAAF1F415FB3D14EE25B2C2F|ganQ]-[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]-[gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]'' mutant is impaired in the utilization of galactan [Pubmed|22893383]

The protein

Catalyzed reaction/ biological activity

• Endohydrolysis of (14)-beta-D-galactosidic linkages in galactan, generation of galactotriose [Pubmed|22893383]

Protein family

• glycosyl hydrolase 53 family (single member, according to UniProt)

Structure

• [PDB|2J74] (B. licheniformis)

[SW|Localization]

• likely to be extracellular, carries a predicted a signal peptide of 26 amino acid residues at the N-terminus [Pubmed|27501980,22893383]

Expression and Regulation

Operons

• genes: [gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]-[gene|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]-[gene|676932E7DF143DC9AAAF1F415FB3D14EE25B2C2F|ganQ]-[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]-[gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]
  description: [Pubmed|9287030]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|27501980], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|1F787F70C87DEB221709579122D63C2322A84E56|GanR]: repression, [Pubmed|9287030], in [regulon|1F787F70C87DEB221709579122D63C2322A84E56|GanR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [pubmed|28617843], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • induced by galactan (binding of galactobiose to [protein|1F787F70C87DEB221709579122D63C2322A84E56|GanR]) [Pubmed|28617843,27501980]
  • repressed by glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|28617843,27501980]

Biological materials

Mutant

• MGNA-A487 (yvfO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/487 NBRP B. subtilis, Japan]
• BKE34120 ([gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTTCATCCCCCCAAG, downstream forward: _UP4_TGAAACAAAAAAATCCATGA
• BKK34120 ([gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTTCATCCCCCCAAG, downstream forward: _UP4_TGAAACAAAAAAATCCATGA

References

• 17056685,9287030,22893383,27501980