srfAD

surfactin synthetase / competence

locus: BSU_03520

pI: 5.22

mw: 27.47 kDa

protein length: 242 aa

gene length: 729 bp

function: antibiotic synthesis

product: surfactin synthetase / competence

essential: no

synonyms: comL

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.4|Swarming]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

Gene

Coordinates: 402,388 403,116

The protein

Protein family

• thioesterase family (with [protein|27C362A132DDFF09E27FC919D0204D4C9E016803|YneP], according to UniProt)

Structure

• [PDB|2RON] [pubmed|18704089]
• [PDB|2K2Q]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|81845F44CE2C601555066E31E87384FF5D7B4139|srfAA]-[gene|3C484FDB3A440269E87D6B6E8500A875AC456666|srfAB]-[gene|4D76F07D6124D56C7CEBBDAB9E84B2718BF1E7FC|comS]-[gene|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|srfAC]-[gene|9D0914FC38FFF4028C2BA5970DC87B3A1027E253|srfAD]
  description: [Pubmed|1715856]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1715856], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|25666134], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|8830686], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]: activation, [Pubmed|1715856,16091051], in [regulon|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA regulon]
  • [protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR]: activation, [Pubmed|16166527], in [regulon|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR regulon]
  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: repression, [Pubmed|12642660], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]
  • [protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|Abh]: repression, [Pubmed|20817675], in [regulon|5872812AB61E92E2944E926915EB7FEE71BFA6D5|Abh regulon]

  regulation

  • expressed at high cell density ([protein|search|ComA]) [Pubmed|16091051]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|hxlR]' and '[protein|search|srfAA]' [PubMed|20525796]

Biological materials

Mutant

• BKE03520 ([gene|9D0914FC38FFF4028C2BA5970DC87B3A1027E253|srfAD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGGCTGTCTCCTCCTTT, downstream forward: _UP4_TGATCAAAAGCGGACAGCTT
• BKK03520 ([gene|9D0914FC38FFF4028C2BA5970DC87B3A1027E253|srfAD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGGCTGTCTCCTCCTTT, downstream forward: _UP4_TGATCAAAAGCGGACAGCTT

References

Reviews

• 20735481

Original publications

• 18704089,16166527,17227471,17190806,8830686,1715856,12642660,16091051,22511326,20817675,15378759