drm

phosphopentomutase

Locus: BSU_23500

Isoelectric point: 4.97

Molecular weight: 43.77 kDa

Protein length: 394 aa

Gene length: 1185 bp

Function: utilization of deoxyribose

Product: phosphopentomutase

Essential: no

E.C.: 5.4.2.7

Synonyms: yqkN

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Utilization of nucleotides
• Groups of genes → Phosphoproteins → Phosphorylation on a Thr residue

Gene

Coordinates: 2,447,246 2,448,430

The protein

Catalyzed reaction/ biological activity

• α-D-ribose 1-phosphate --> D-ribose 5-phosphate (according to UniProt)
• 2-deoxy-α-D-ribose 1-phosphate --> 2-deoxy-D-ribose 5-phosphate (according to UniProt)

Protein family

• phosphopentomutase family (single member, according to UniProt)

Modification

• phosphorylation on (Thr-87 OR Thr-89) PubMed

Structure

• 3M8W (from B. cereus, 77% identity, 86% similarity) PubMed

Expression and Regulation

Operons

• Genes: drm-pupG
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • induced in the presence of nucleosides (deoxyribose 5-phosphate and ribose 5-phosphate act as molecular inducers) PubMed

Biological materials

Mutant

• BKE23500 (drm::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTGAAAGCCTCCTTTT, downstream forward: _UP4_TAGGGGGACTGTTTCTTGAA
• BKK23500 (drm::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTGAAAGCCTCCTTTT, downstream forward: _UP4_TAGGGGGACTGTTTCTTGAA

References

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