cotC

spore coat protein (outer)

locus: BSU_17700

pI: 9.56

mw: 14.64 kDa

protein length: 66 aa

gene length: 201 bp

function: resistance of the spore

product: spore coat protein (outer)

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.8|Not yet assigned]

Gene

Coordinates: 1,904,995 1,905,195

The protein

Paralogous protein(s)

• [protein|EB690FA075EAC3E298C47AED082B5FC21E8F8F46|CotU]

[SW|Localization]

• outer spore coat, more abundant at the mother cell-distal pole of the forespore [Pubmed|19933362]
• the [protein|9C59B51FC19FC83BD14272008BD441833DD1738E|CotC]-[protein|EB690FA075EAC3E298C47AED082B5FC21E8F8F46|CotU] complex assembles around the forming spore [Pubmed|18065538]

Expression and Regulation

Operons

• genes: [gene|9C59B51FC19FC83BD14272008BD441833DD1738E|cotC]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|1518043], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|1518043], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]
  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: repression, [Pubmed|10788508], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK], [protein|search|GerE], [SW|SpoIIID]) [Pubmed|1518043,10788508]

Biological materials

Mutant

• 1S103 ( ''cotC''::''cat''), [Pubmed|2821284], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S103&Search=1S103 BGSC]
• BKE17700 ([gene|9C59B51FC19FC83BD14272008BD441833DD1738E|cotC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATACTCCTCCTTTAT, downstream forward: _UP4_TAAACGCCATTAACATCTCC
• BKK17700 ([gene|9C59B51FC19FC83BD14272008BD441833DD1738E|cotC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATACTCCTCCTTTAT, downstream forward: _UP4_TAAACGCCATTAACATCTCC

References

Reviews

• 27227299

Original Publications

• 19933362,1518043,2821284,12031481,1691789,18065538,10788508,20023017,15470121,26953338