ileS [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

isoleucyl-tRNA synthetase

locus: BSU15430

pI: 5.00

mw: 104.00 kDa

function: translation

product: isoleucyl-tRNA synthetase

essential: yes

ec: 6.1.1.5

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

[SW|Categories] containing this gene/protein

• [SW|translation], [SW|essential genes]

This gene is a member of the following [SW|regulons]

• [SW|T-box]

Gene

Coordinates on the chromosome (coding sequence): 1,613,357 -> 1,616,122

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP L-isoleucine tRNA(Ile) = AMP diphosphate L-isoleucyl-tRNA(Ile) (according to Swiss-Prot)

Protein family

• IleS type 1 subfamily (according to Swiss-Prot)

Structure

• [PDB|1QU2] (from ''Staphylococcus aureus'', 61% identity) [Pubmed|10446055]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• :
• subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=17981983 PubMed]

Expression and Regulation

Regulation

• induced by isoleucine limitation ([protein|search|T-box]) [Pubmed|19258532]

Regulatory mechanism

• [protein|search|T-box]: [SW|RNA switch], transcriptional antitermination [Pubmed|19258532]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=17981983 PubMed]

References

• 19258532,10446055,17981983,19233850