SubtiBank SubtiBank
ileS
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ileS

isoleucyl-tRNA synthetase
locus
BSU_15430
pI
5.19
mw
104.58 kDa
protein length
921 aa Sequence Blast
gene length
2766 bp Sequence Blast
function
translation
product
isoleucyl-tRNA synthetase
essential
yes
ec
6.1.1.5
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    1,613,357 1,616,122

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP + L-isoleucine + tRNAIle --> AMP + diphosphate + L-isoleucyl-tRNAIle (according to UniProt)

    • Protein family

  • [SW|Class-I aminoacyl-tRNA synthetase family] (according to UniProt)

    • Structure

  • [PDB|1QU2] (from ''Staphylococcus aureus'', 61% identity) [Pubmed|10446055]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

    • Expression and Regulation

    Operons

    genes
    [gene|9C29039BB3EE70FEC91C0F0919E579E76EEB193A|ileS]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [regulon|T-box|T-box]: antitermination, [pubmed|19258532], in [regulon|T-box|T-box]

    • regulation

  • induced by isoleucine limitation ([SW|T-box]) [Pubmed|19258532]
  • the [SW|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
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    References

  • 19258532,10446055,17981983,19233850,28139725,29794222