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purD [2019-07-23 11:46:24]
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purD [2019-07-23 11:46:24]

phosphoribosylglycinamide synthetase
Locus
BSU_06530
Isoelectric point
4.60
Molecular weight
45.12 kDa
Protein length
422 aa Sequence Blast
Gene length
1269 bp Sequence Blast
Function
purine biosynthesis
Product
phosphoribosylglycinamide synthetase
Essential
no
E.C.
6.3.4.13
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of purine nucleotides

    • Gene

    Coordinates
    710,148 711,416

    The protein

    Catalyzed reaction/ biological activity

  • ATP 5-phospho-D-ribosylamine glycine = ADP phosphate N(1)-(5-phospho-D-ribosyl)glycinamide (according to Swiss-Prot)

    • Protein family

  • GARS family (single member, according to UniProt)

    • Structure

  • 2XCL

    • Additional information

  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • G-box: termination, (riboswitch) PubMed, in G-box
  • PurR: repression, (molecular inducer: PRPP) PubMed, in PurR regulon

    • Regulation

  • expression activated by glucose (4.4 fold) PubMed
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    Biological materials

    Mutant

  • BKE06530 (purD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TACATTCACGTCGTTTTCAT, downstream forward: _UP4_TAAGTGAGGAAAACCCGCAG
  • BKK06530 (purD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TACATTCACGTCGTTTTCAT, downstream forward: _UP4_TAAGTGAGGAAAACCCGCAG

    • References

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