purD

phosphoribosylglycinamide synthetase

Locus: BSU_06530

Isoelectric point: 4.60

Molecular weight: 45.12 kDa

Protein length: 422 aa

Gene length: 1269 bp

Function: purine biosynthesis

Product: phosphoribosylglycinamide synthetase

Essential: no

E.C.: 6.3.4.13

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of purine nucleotides

Gene

Coordinates: 710,148 711,416

The protein

Catalyzed reaction/ biological activity

• 5-phospho-D-ribosylamine + ATP + glycine --> ADP + H⁺ + N¹-(5-phospho-D-ribosyl)glycinamide + phosphate (according to UniProt)

Protein family

• GARS family (single member, according to UniProt)

Domains

• ATP-grasp doamin (aa 107-313) (according to UniProt)

Structure

• 2XCL

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • G-box: termination, (riboswitch) PubMed, in G-box
  • PurR: repression, (molecular inducer: PRPP) PubMed, in PurR regulon

  Regulation

  • expression activated by glucose (4.4 fold) PubMed

Biological materials

Mutant

• BKE06530 (purD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TACATTCACGTCGTTTTCAT, downstream forward: _UP4_TAAGTGAGGAAAACCCGCAG
• BKK06530 (purD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TACATTCACGTCGTTTTCAT, downstream forward: _UP4_TAAGTGAGGAAAACCCGCAG

References

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