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gdpP [2020-07-01 16:43:32]
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gdpP [2020-07-01 16:43:32]

c-di-AMP specific phosphodiesterase
Locus
BSU_40510
Isoelectric point
4.86
Molecular weight
74.14 kDa
Protein length
659 aa Sequence Blast
Gene length
1980 bp Sequence Blast
Function
control of sporulation initiation, cell wall homeostasis
Product
c-di-AMP phosphodiesterase
Essential
no
Synonyms
yybT
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Metabolism of signalling nucleotides
  • Information processing → Targets of second messengers → Targets of (p)ppGpp
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    4,163,643 4,165,622

    Phenotypes of a mutant

  • inactivation of gdpP strongly restores beta-lactam resistance in a sigM mutant PubMed
  • a gdpP pgpH double mutant acquires suppressor mutations in cdaA PubMed
  • a gdpP pgpH double mutant is defective in biofilm formation PubMed
  • altered morphology on MSgg medium PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • cyclic dinucleotide phosphodiesterase, hydrolysis of cyclic di-AMP PubMed
  • 3',3'-c-di-AMP + H2O --> 5'-O-phosphonoadenylyl-(3'→5')-adenosine + H+ (according to UniProt)

    • Protein family

  • GdpP/PdeA phosphodiesterase family (single member, according to UniProt)

    • Domains

  • two transmembrane domains at the N-terminus PubMed
  • a domain that shares minimum sequence homology with Heme-binding [PAS domain|Per-ARNT-Sim (PAS) domains] (80 aa) PubMed
  • degenerate GGDEF domain (aa 173 - 301) PubMed
  • a DHH-DHHA1 domain PubMed

    • Effectors of protein activity

  • ppGpp inhibits cyclic dinucleotide phosphodiesterase activity PubMed
  • Heme binding suppresses phosphodiesterase activity PubMed

    • Structure

  • 2M1C (N-terminal PAS domain of GdpP from Geobacillus thermodenitrificans) PubMed

    • Localization

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    Genes
    yybS-gdpP-rplI
    Description
    PubMed

    Additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of yybS PubMed
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    Biological materials

    Mutant

  • MGNA-B834 (yybT::erm), available at the NBRP B. subtilis, Japan
  • GP998 (gdpP::spc), available in Jörg Stülke's lab PubMed
  • GP2040 (gdpP::spc pgpH::erm without terminator, available in Jörg Stülke's lab) PubMed
  • BKE40510 (gdpP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCTATCACTCCCCACC, downstream forward: _UP4_AGATGAAGGTTATTTTCTTA
  • BKK40510 (gdpP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCTATCACTCCCCACC, downstream forward: _UP4_AGATGAAGGTTATTTTCTTA

    • Labs working on this gene/protein

  • Jörg Stülke, University of Göttingen, Germany Homepage

    • References

    Reviews

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    Original publications

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    GdpP homologs in other organisms

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