yumB

similar to type II NADH dehydrogenase (non proton-pumping)

locus: BSU_32100

pI: 6.62

mw: 44.77 kDa

protein length: 406 aa

gene length: 1221 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]

Gene

Coordinates: 3,300,034 3,301,254

The protein

Protein family

• NADH dehydrogenase family (with [protein|5044FDBEC8D81D1978DB431F9222902FCD80DD2D|YutJ] and [protein|56F407D408272F3674612C9CDFE4A922680EC694|Ndh], according to UniProt)

Paralogous protein(s)

• [protein|56F407D408272F3674612C9CDFE4A922680EC694|Ndh]

[SW|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|4NWZ] (from Caldalkalibacillus thermarum, 62% identity) [pubmed|24444429]

Expression and Regulation

Operons

• genes: [gene|9B3E9074975758BCB3D06820E4A35F8C2B78DCEB|yumB]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B571 (yumB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1570 NBRP B. subtilis, Japan]
• BKE32100 ([gene|9B3E9074975758BCB3D06820E4A35F8C2B78DCEB|yumB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACATCCACCTTCTCTT, downstream forward: _UP4_TAAAAACCCGCGGAAGCGGG
• BKK32100 ([gene|9B3E9074975758BCB3D06820E4A35F8C2B78DCEB|yumB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACATCCACCTTCTCTT, downstream forward: _UP4_TAAAAACCCGCGGAAGCGGG

References

• 17015645,19935659,24444429