bmrD

multidrug ABC transporter (ATP-binding protein), also involved in the signalling pathway to activate KinA at the onset of sporulation

Locus: BSU_09720

Isoelectric point: 7.08

Molecular weight: 76.12 kDa

Protein length: 673 aa

Gene length: 2022 bp

Function: multiple antibiotic resistance

Product: multidrug ABC transporter (ATP-binding protein)

Essential: no

Synonyms: yheH

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Exporters → Efflux of antibiotics
• Cellular processes → Transporters → ABC transporters → Regulatory ABC transporters
• Lifestyles → Coping with stress → Resistance against toxins/ antibiotics
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,047,072 1,049,093

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Paralogous protein(s)

• YgaD

Domains

• has both a membrane-spanning and an ATP-binding domain PubMed
• ABC transmembrane type-1 domain (aa 18-398) (according to UniProt)
• ABC transporter domain (aa 430-664) (according to UniProt)

Structure

• 5MKK (TmrAB complex from Thermus thermophilus, 38% identity) PubMed

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: bmrB-bmrC-bmrD
  Description: PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon
  • BmrB: attenuation, PubMed, in BmrB regulon

  Regulation

  • bmrB-bmrC-bmrD expression only occurs during the late-exponential and stationary growth stages (AbrB) PubMed

Biological materials

Mutant

• MGNA-B491 (yheH::erm), available at the NBRP B. subtilis, Japan
• BKE09720 (bmrD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCTCCATAACGTTTTTCCTA, downstream forward: _UP4_TAACGCTCAAAAACCCAAAA
• BKK09720 (bmrD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCTCCATAACGTTTTTCCTA, downstream forward: _UP4_TAACGCTCAAAAACCCAAAA

References

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