SubtiBank SubtiBank
yrdN [2018-02-10T13:17:17.000Z]
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yrdN [2018-02-10T13:17:17.000Z]

putative 4-oxalocrotonate tautomerase
locus
BSU26660
pI
5.01
mw
14.54 kDa
protein length
129 aa Sequence Blast
gene length
387 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
  • Gene

    Coordinates
    2,725,114 → 2,725,503

    The protein

    Protein family

  • 4-oxalocrotonate tautomerase family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|1F6B2CF88F5C6498A035FA0465136EF9D0BF93BC|YodA] (67,4%)
  • Structure

  • [PDB|2AAG] (from Pseudomonas pavonaceae 54% identity)
  • [SW|Localization]

  • cell membrane (according to Swiss-Prot)
  • Biological materials

    Mutant

  • MGNA-A862 (yrdN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/862 NBRP B. subtilis, Japan]
  • BKE26660 (Δ[gene|9A56CC9B3C33D74A7349112930ADF627099DA907|yrdN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE26660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAATCCCCTCCTTCTA, downstream forward: _UP4_TAGTTGAATACTACCCTCAA
  • BKK26660 (Δ[gene|9A56CC9B3C33D74A7349112930ADF627099DA907|yrdN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK26660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAATCCCCTCCTTCTA, downstream forward: _UP4_TAGTTGAATACTACCCTCAA