SubtiBank SubtiBank
ykkE
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ykkE

formyltetrahydrofolate deformylase
Locus
BSU_13110
Isoelectric point
5.95
Molecular weight
34.34 kDa
Protein length
300 aa Sequence Blast
Gene length
903 bp Sequence Blast
Function
purine nucleotide synthesis
Product
formyltetrahydrofolate deformylase
Essential
no
E.C.
3.5.1.10
Synonyms
purU

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,377,243 1,378,145

The protein

Catalyzed reaction/ biological activity

  • (6S)-10-formyltetrahydrofolate + H2O --> (6S)-5,6,7,8-tetrahydrofolate + formate + H+ (according to UniProt)
  • Protein family

  • PurU family (single member, according to UniProt)
  • Paralogous protein(s)

    Domains

  • ACT domain (aa 21-102) (according to UniProt)
  • Structure

  • 3W7B (from Thermus thermophilus, 58% identity) PubMed
  • Expression and Regulation

    Operons

    Biological materials

    Mutant

  • MGNA-B310 (ykkE::erm), available at the NBRP B. subtilis, Japan
  • BKE13110 (ykkE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAATAATCCCTCTTATC, downstream forward: _UP4_TAGACTGCAAGAGGCCCGCG
  • BKK13110 (ykkE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAATAATCCCTCTTATC, downstream forward: _UP4_TAGACTGCAAGAGGCCCGCG
  • References

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