liaS [2020-10-31 16:27:36]

two-component sensor kinase/phosphatase, response to bacitracin

locus: BSU_33090

pI: 7.33

mw: 40.55 kDa

protein length: 360 aa

gene length: 1083 bp

function: control of [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR] activity in response to bacitracin

product: two-component sensor kinase

essential: no

synonyms: yvqE

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 3,395,035 3,396,117

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR]
• dephosphorylation of [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR] in the absence of the stress signal [Pubmed|23279150]
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

[SW|Domains]

• two transmembrane segments
• [SW|HAMP domain] (aa 74-126) (according to UniProt)
• [SW|Histidine kinase domain] (aa 153-346) (according to UniProt)

Modification

• autophosphorylation on a His residue

Effectors of protein activity

• LiaS kinase activity is inhibited by [protein|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|LiaF], phosphatase acitivity is maintained by [protein|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|LiaF] in the absence of the stress signal [Pubmed|23279150]

Structure

• [PDB|3GIG] ([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|DesK], corresponds to the C-terminal domain, aa 149 ... 343, 25% identity) [pubmed|19805278]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|A71B0392FFF70EFFC096E7E9BD5FE30792821CDD|liaG]-[gene|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|liaF]-[gene|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]-[gene|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]
  description: [Pubmed|16816187]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15273097], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR]: activation, [Pubmed|16816187], in [regulon|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR regulon]

  regulation

  • ''[protein|search|liaG]'': constitutive

• genes: [gene|B6D1159454969D1D578E05D5CE2259E079688510|liaI]-[gene|AE4BF12C368468C553EED7A696D5EFC63F56CA39|liaH]-[gene|A71B0392FFF70EFFC096E7E9BD5FE30792821CDD|liaG]-[gene|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|liaF]-[gene|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]-[gene|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]
  description: [Pubmed|14651641]

  regulation

  • ''[protein|search|liaG]'': constitutive

Biological materials

Mutant

• MGNA-B036 (yvqE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1035 NBRP B. subtilis, Japan]
• BKE33090 ([gene|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAGGCTGGCAAGCATTTTTT, downstream forward: _UP4_CCGGAAGAAAAAGGAGAGAA
• BKK33090 ([gene|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAGGCTGGCAAGCATTTTTT, downstream forward: _UP4_CCGGAAGAAAAAGGAGAGAA

labs

• [SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]

References

Reviews

• 27344142

Original publications

• 18394148,19164152,15273097,17921301,10094672,19164157,14651641,17600057,17660417,16816187,15101989,17660417,20057163,23279150,20817675,22964256,22092710,23326432,27856233,29185696,19805278,30848888,32117169,32349670