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liaS [2019-06-06 09:45:03]
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liaS [2019-06-06 09:45:03]

two-component sensor kinase/phosphatase, response to bacitracin
Locus
BSU33090
Isoelectric point
7.33
Molecular weight
40.55 kDa
Protein length
360 aa Sequence Blast
Gene length
1083 bp Sequence Blast
Function
control of LiaR activity in response to bacitracin
Product
two-component sensor kinase
Essential
no
Synonyms
yvqE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Protein modification → Protein kinases
  • Information processing → Regulation of gene expression → Transcription factors and their control → Control of two-component response regulators → Two-component sensor kinase
  • Lifestyles → Coping with stress → Resistance against toxins/ antibiotics
  • Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress
  • Groups of genes → Membrane proteins
  • Groups of genes → Phosphoproteins → Phosphorylation on a His residue

    • Gene

    Coordinates
    3,395,035 3,396,117

    The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of LiaR
  • dephosphorylation of LiaR in the absence of the stress signal PubMed

    • Domains

  • two transmembrane segments, C-terminal histidine phosphotransferase domain

    • Modification

  • autophosphorylation on a His residue

    • Effectors of protein activity

  • LiaS kinase activity is inhibited by LiaF, phosphatase acitivity is maintained by LiaF in the absence of the stress signal PubMed

    • Structure

  • 3GIG (DesK, corresponds to the C-terminal domain, aa 149 ... 343, 25% identity) PubMed

    • Localization

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    liaG-liaF-liaS-liaR
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • LiaR: activation, PubMed, in LiaR regulon

    • Regulation

  • liaG: constitutive
    • view in new tab

    Genes
    liaI-liaH-liaG-liaF-liaS-liaR
    Description
    PubMed

    Regulation

  • liaG: constitutive
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B036 (yvqE::erm), available at the NBRP B. subtilis, Japan
  • BKE33090 (liaS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GAGGCTGGCAAGCATTTTTT, downstream forward: _UP4_CCGGAAGAAAAAGGAGAGAA
  • BKK33090 (liaS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GAGGCTGGCAAGCATTTTTT, downstream forward: _UP4_CCGGAAGAAAAAGGAGAGAA

    • Labs working on this gene/protein

  • John Helmann, Cornell University, USA Homepage

    • References

    Reviews

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    Original publications

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