pelC

pectate lyase C

locus: BSU_34950

pI: 9.14

mw: 24.13 kDa

protein length: 221 aa

gene length: 666 bp

function: degradation of polygalacturonic acid

product: pectate lyase C

essential: no

ec: 4.2.2.2

synonyms: yvpA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 3,590,603 3,591,268

The protein

Catalyzed reaction/ biological activity

• Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends (according to UniProt)

Protein family

• polysaccharide lyase 3 family (single member, according to UniProt)

Structure

• [PDB|1EE6] (from Bacillus sp., 58% identity) [pubmed|11717490]

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|950D355D993DF79AD5288A00AA04A775637AC04D|yvpB]-[gene|99ECA1B305145A63BD3A72094EF24F56D875BCC1|pelC]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A382 (yvpA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/382 NBRP B. subtilis, Japan]
• BKE34950 ([gene|99ECA1B305145A63BD3A72094EF24F56D875BCC1|pelC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGATAAATCCTCCCCGG, downstream forward: _UP4_TTTTAACAAAAAAAGTCCGC
• BKK34950 ([gene|99ECA1B305145A63BD3A72094EF24F56D875BCC1|pelC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGATAAATCCTCCCCGG, downstream forward: _UP4_TTTTAACAAAAAAAGTCCGC

References

• 18957862,16514142,20525796,24236123,11717490