nucA

membrane-associated nuclease, catalyzes DNA cleavage during transformation

locus: BSU_03430

pI: 4.68

mw: 16.12 kDa

protein length: 147 aa

gene length: 444 bp

function: genetic transformation, DNA uptake

product: membrane-associated nuclease

essential: no

synonyms: comI

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 372,154 372,597

The protein

Structure

• [PDB|5OMT] ([protein|search|NucB ]from B. licheniformis, 64% identity, aa 40 - 147) [pubmed|29165717]

[SW|Localization]

• integral membrane protein [Pubmed|11359569]

Expression and Regulation

Operons

• genes: [gene|99581CC6AFFA2D1392748B4D9C99DEF8B7601F75|nucA]-[gene|3A54C644DD1CCA5C08CB9C5CD648D44889E68D23|nin]
  description: [Pubmed|11359569]

  regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|7746143], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]

Biological materials

Mutant

• BKE03430 ([gene|99581CC6AFFA2D1392748B4D9C99DEF8B7601F75|nucA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGTTCACCTCCCGCTTTT, downstream forward: _UP4_TAAACAGTACATAGAGGAGG
• BKK03430 ([gene|99581CC6AFFA2D1392748B4D9C99DEF8B7601F75|nucA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGTTCACCTCCCGCTTTT, downstream forward: _UP4_TAAACAGTACATAGAGGAGG

References

• 11814663,11359569,7746143,29165717