SubtiBank SubtiBank
pucB
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pucB

xanthine dehydrogenase
Locus
BSU_32500
Isoelectric point
7.92
Molecular weight
20.91 kDa
Protein length
205 aa Sequence Blast
Gene length
618 bp Sequence Blast
Function
purine utilization
Product
xanthine dehydrogenase
Essential
no
Synonyms
yurE

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,339,331 3,339,948

The protein

Structure

  • 2WAW (from Mycobacterium tuberculosis, 27% identity)
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • PucR: activation, PubMed, in PucR regulon
  • Regulation

  • induced in the presence of purine nucleotides (effector: allantoin) (PucR) PubMed
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    Biological materials

    Mutant

  • MGNA-A941 (yurE::erm), available at the NBRP B. subtilis, Japan
  • BKE32500 (pucB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCTCATACAACCGCCACCCG, downstream forward: _UP4_TCTTGAACGGCCAAGTGACA
  • BKK32500 (pucB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCTCATACAACCGCCACCCG, downstream forward: _UP4_TCTTGAACGGCCAAGTGACA
  • References

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