ycnK [2019-09-03 11:07:00]

copper-responsive transcription repressor of the ycnK-ycnJ-ycnI operon, DeoR family

Locus: BSU_03960

Isoelectric point: 7.37

Molecular weight: 21.29 kDa

Protein length: 190 aa

Gene length: 573 bp

Function: regulation of copper uptake

Product: transcription repressor, DeoR family

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Homeostasis → Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo) → Copper
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other

Gene

Coordinates: 448,461 449,033

Phenotypes of a mutant

• elevated expression of ycnJ under conditions of copper excess PubMed

The protein

Catalyzed reaction/ biological activity

• repression of ycnK-ycnJ-ycnI expression in the presence of excess copper PubMed

Protein family

• DeoR family of transcription factors

Domains

• HTH deoR-type domain (aa 3-58) (according to UniProt)

Cofactors

• copper acts as corepressor PubMed

Expression and Regulation

Operons

• Genes: ycnK-ycnJ-ycnI
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • YcnK: repression, PubMed, in YcnK regulon
  • AbrB: repression, PubMed, in AbrB regulon

  Regulation

  • induced by copper limitation (YcnK) PubMed

Biological materials

Mutant

• MGNA-C017 (ycnK::erm), available at the NBRP B. subtilis, Japan
• BKE03960 (ycnK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCATACACCCTCTTCAT, downstream forward: _UP4_TAACACAACACATACAGCGG
• BKK03960 (ycnK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCATACACCCTCTTCAT, downstream forward: _UP4_TAACACAACACATACAGCGG

Labs working on this gene/protein

• Mohamed Marahiel, Marburg University, Germany homepage

References

Reviews

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Original publications

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