spo0F

phosphotransferase of the [SW|sporulation] initiation [SW|phosphorelay]

locus: BSU_37130

pI: 4.70

mw: 14.09 kDa

protein length: 124 aa

gene length: 375 bp

function: initiation of [SW|sporulation]

product: phosphotransferase of the sporulation initiation phosphorelay

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.3|The phosphotransferases]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.3|The phosphotransferases]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

Gene

Coordinates: 3,809,550 3,809,924

The protein

[SW|Domains]

• [SW|Response regulatory domain] (aa 5-119) (according to UniProt)

Modification

• phosphorylated on an Asp residue by [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|KinA], [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|KinB], [protein|A656321846B2E0D1F39B528E2D8B8E620CCD1148|KinC], [protein|511E71BB1981758857854C8E9BF657287CE60C11|KinD], or [protein|1582E81F7DA85F38D6732D341ABD0F052587F25A|KinE]
• dephosphorylated by [protein|25D89EF3C4D57B3E6F9AB0210029651F74356906|RapA], [protein|E07E123CC9FB3B29DDA8EC58A33FEEE1DDFC4834|RapB], [protein|8B8646DF7F437D8DB299A77BA937DC6FC082102F|RapE], or [protein|BA85E2C2E2A6B76FAFA31EBDBF466324C9B021C8|RapH] [Pubmed|17581123]

Structure

• [PDB|1SRR] (phosphatase resistant mutant)
• [PDB|1F51] (complex with [protein|29D91115BB3AC03538D618E54A5A57F92777EFF6|Spo0B])
• [PDB|2JVK] (Mutant L66A),
• [PDB|2FSP]
• [PDB|3Q15] ([protein|BA85E2C2E2A6B76FAFA31EBDBF466324C9B021C8|RapH]-[protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F] complex) [Pubmed|21346797]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F]
  description: [Pubmed|2457578]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2457578], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|1569009], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|8483422], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|8483422]

Biological materials

Mutant

• BKE37130 ([gene|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTACACCCCAATATTA, downstream forward: _UP4_TGACAAAAAGAAGAAACAAA
• BKK37130 ([gene|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTACACCCCAATATTA, downstream forward: _UP4_TGACAAAAAGAAGAAACAAA

References

Reviews

• 28886686

Original Publications

• 20413551,9299348,9335530,11923303,8608130,16788205,12067336,8497199,10094672,15299688,8643670,8805550,26165942,11069677,2997779,8483422,16333746,2118512,10997904,9601032,9254596,8730857,10411754,17350627,21346797,9341920,9843420,2509430,1846779,9540996,9477965,17581123,8483422,2457578,1569009,23490197,21346797,22267516,23524609,25598361,27501195,30125296,30212463