spo0F

phosphotransferase of the sporulation initiation phosphorelay

Locus: BSU_37130

Isoelectric point: 4.70

Molecular weight: 14.09 kDa

Protein length: 124 aa

Gene length: 375 bp

Function: initiation of sporulation

Product: phosphotransferase of the sporulation initiation phosphorelay

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → phosphorelay → The phosphotransferases
• Lifestyles → Sporulation → phosphorelay → The phosphotransferases
• Groups of genes → Phosphoproteins → Phosphorylation on an Asp residue

Gene

Coordinates: 3,809,550 3,809,924

The protein

Domains

• Response regulatory domain (aa 5-119) (according to UniProt)

Modification

• phosphorylated on an Asp residue by KinA, KinB, KinC, KinD, or KinE
• dephosphorylated by RapA, RapB, RapE, or RapH PubMed

Structure

• 1SRR (phosphatase resistant mutant)
• 1F51 (complex with Spo0B)
• 2JVK (Mutant L66A),
• 2FSP
• 3Q15 (RapH-Spo0F complex) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: spo0F
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigH: sigma factor, PubMed, in SigH regulon

  Regulatory mechanism

  • Spo0A: activation, PubMed, in Spo0A regulon

  Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed

Biological materials

Mutant

• BKE37130 (spo0F::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTACACCCCAATATTA, downstream forward: _UP4_TGACAAAAAGAAGAAACAAA
• BKK37130 (spo0F::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTACACCCCAATATTA, downstream forward: _UP4_TGACAAAAAGAAGAAACAAA

References

Reviews

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Original Publications

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