psdR

two-component response regulator (OmpR family), induction of psdA-psdB in response to lipid II-binding lantibiotics (nisin and gallidermin) and internal antimicrobial peptides (SdpC, SkfA)

Locus: BSU_34720

Isoelectric point: 4.68

Molecular weight: 27.37 kDa

Protein length: 237 aa

Gene length: 714 bp

Function: resistance against toxic peptides

Product: two-component response regulator (OmpR family)

Essential: no

Synonyms: yvcP

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Two-component system response regulators
• Lifestyles → Coping with stress → Resistance against toxins/ antibiotics
• Groups of genes → Phosphoproteins → Phosphorylation on an Asp residue

Gene

Coordinates: 3,567,422 3,568,135

The protein

Protein family

• OmpR family of two-component response regulators

Domains

• Response regulatory domain (aa 3-116) (according to UniProt)

Modification

• phosphorylated by PsdS on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Structure

• 5DCL (from Streptococcus agalactiae, 42% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: psdR-psdS-psdA-psdB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • PsdR: activation, PubMed, in PsdR regulon

  Regulation

  • psdA: induced by nisin, gallidermin (PsdR) PubMed

Biological materials

Mutant

• MGNA-B637 (yvcP::erm), available at the NBRP B. subtilis, Japan
• BKE34720 (psdR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGTTGATCCCCCTTATAT, downstream forward: _UP4_GTCAATCGGAAGGATGAAGC
• BKK34720 (psdR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGTTGATCCCCCTTATAT, downstream forward: _UP4_GTCAATCGGAAGGATGAAGC

References

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