yfnG [2018-02-12T16:26:50.000Z]

similar to CDP-glucose 4,6-dehydratase

locus: BSU07280

pI: 5.34

mw: 33.96 kDa

protein length: 301 aa

gene length: 903 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 799,303 → 800,208

The protein

Protein family

• sugar epimerase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|90D505CAA4FAAEB6A6AF5C278E70DF714FACB4DA|SpsJ] (31%), [protein|A51B5F9F86D2F7B690712B9EC181B1C07B312E02|YtcB] (29,5%)

Structure

• [PDB|4EGB] (from ''Bacillus Anthracis Str.Ames'', 31% identity)

Expression and Regulation

Operons

• genes: [gene|B25AE6979AF12628780C4489FFED966C6B31968A|yfnH]-[gene|9836E90BD443F5269A43E5C69FCF2CCB22EDF65B|yfnG]-[gene|4148A3F8E256D9B1D667C28428D867B5B6B5F3CE|yfnF]-[gene|E3481379D3FEC02C35CB4BE17A5246A208C5C86D|yfnE]-[gene|1D0286ECDA32FB21737170B54A57D3FE482C6422|yfnD]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15383836], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, internal promoter in [protein|E3481379D3FEC02C35CB4BE17A5246A208C5C86D|YfnE] open reading frame [Pubmed|15383836], see [http://dbtbs.hgc.jp/COG/prom/yfnHGFED.html DBTBS] for details, in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|26577401], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

  regulation

  • expressed during sporulation ([protein|search|SigK]) [Pubmed|15383836]

Biological materials

Mutant

• MGNA-C325 (yfnG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2323 NBRP B. subtilis, Japan]
• BKE07280 (Δ[gene|9836E90BD443F5269A43E5C69FCF2CCB22EDF65B|yfnG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTTTTCCAGAAACTCAAAT, downstream forward: _UP4_TAATGTGAAACGAGGTGAAC
• BKK07280 (Δ[gene|9836E90BD443F5269A43E5C69FCF2CCB22EDF65B|yfnG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTTTTCCAGAAACTCAAAT, downstream forward: _UP4_TAATGTGAAACGAGGTGAAC

References

• 15383836,26577401