thiD

4-amino-5-hydroxymethyl-2-methylpyrimidine and 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate kinase

Locus: BSU_11710

Isoelectric point: 5.71

Molecular weight: 28.97 kDa

Protein length: 271 aa

Gene length: 816 bp

Function: biosynthesis of thiamine pyrophosphate (TPP)

Product: 4-amino-5-hydroxymethyl-2-methylpyrimidinepyrophosphate kinase

Essential: no

E.C.: 2.7.1.49

Synonyms: yjbV

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis/ acquisition of thiamine

Gene

Coordinates: 1,246,837 1,247,652

The protein

Catalyzed reaction/ biological activity

• 4-amino-5-hydroxymethyl-2-methylpyrimidine + ATP --> 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ADP + H⁺ (according to UniProt)
• 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ATP --> 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + ADP (according to UniProt)

Protein family

• ThiD family (with PdxK, according to UniProt)

Paralogous protein(s)

• PdxK

Structure

• 2I5B PubMed

Expression and Regulation

Operons

• Genes: tenA-tenI-thiO-thiS-thiG-thiF-thiD
  Description: PubMed

  Regulatory mechanism

  • Thi-box: RNA switch, via RNA switch, in Thi-box regulon

  Regulation

  • repressed by thiamine (Thi-box) PubMed
  • the Thi-box RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• MGNA-B167 (yjbV::erm), available at the NBRP B. subtilis, Japan
• BKE11710 (thiD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACTCATTACACTACACTCCT, downstream forward: _UP4_TGAGATCACCAGCTGATGGT
• BKK11710 (thiD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACTCATTACACTACACTCCT, downstream forward: _UP4_TGAGATCACCAGCTGATGGT

References

Reviews

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Original publications

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