trpP

S protein of tryptophan ECF transporter

Locus: BSU_10010

Isoelectric point: 11.15

Molecular weight: 18.13 kDa

Protein length: 172 aa

Gene length: 519 bp

Function: tryptophan uptake

Product: S protein of tryptophan ECF transporter

Essential: no

Synonyms: yhaG

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ECF transporter
• Cellular processes → Transporters → ECF transporter → The substrate-specific S components of the ECF transporters
• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of aromatic amino acids
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,074,646 1,075,164

The protein

Protein family

• vitamin uptake transporter (VUT/ECF) (TC 2.A.88) family (with ThiT and YpdP, according to UniProt)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: trpP
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulation

  • induced in the absence of tryptophan (MtrB) PubMed

• Genes: serC-trpP
  Description: PubMed

Other regulations

• MtrB: translation inhibition, at an RNA switch, the MtrB binding site overlaps the Shine-DAlgarno sequence and translation initation region, MtrB binds in the presence of tryptophan PubMed

Biological materials

Mutant

• MGNA-A686 (yhaG::erm), available at the NBRP B. subtilis, Japan
• BKE10010 (trpP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACTATGCTCTCCTCTG, downstream forward: _UP4_TAATTGTCACACAAAACCTC
• BKK10010 (trpP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACTATGCTCTCCTCTG, downstream forward: _UP4_TAATTGTCACACAAAACCTC

References

Reviews

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Original publications

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