pbuE [2019-06-06 11:12:42]

putative hypoxanthine exporter

locus: BSU05800

pI: 8.88

mw: 41.38 kDa

protein length: 388 aa

gene length: 1167 bp

function: nucleotide metabolism

product: putative hypoxanthine exporter

essential: no

synonyms: ydhL

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.6|Nucleotide/ nucleoside transporter]
• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.4|Nucleotide metabolism/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 625,125 626,291

The protein

Protein family

• [SW|major facilitator superfamily] (according to UniProt)

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|96B48D9B984010FECD2C194D7CF3CADFC22F2AA7|pbuE]
  description: [pubmed|22383849]

  regulatory mechanism

  • [regulon|a-box|a-box]: termination/antitermination, adenine-controlled, in [regulon|a-box|a-box]

  regulation

  • induced by hypoxanthine and guanine, [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR]-independent [Pubmed|12923093]
  • induced by adenine ([regulon|A-box|A-box]) [Pubmed|14718920]

Biological materials

Mutant

• MGNA-C187 (ydhL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2185 NBRP B. subtilis, Japan]
• BKE05800 ([gene|96B48D9B984010FECD2C194D7CF3CADFC22F2AA7|pbuE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACAAACTCCTTTACTT, downstream forward: _UP4_TCCTTGTAAAAGGAGGATTT
• BKK05800 ([gene|96B48D9B984010FECD2C194D7CF3CADFC22F2AA7|pbuE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACAAACTCCTTTACTT, downstream forward: _UP4_TCCTTGTAAAAGGAGGATTT

References

• 25550163,15629952,14718920,12923093,12923093,18441052,17935948,17853814,16931335,16201765,20022916,12787499,21283784,25573585