pbuE

putative hypoxanthine exporter

Locus: BSU_05800

Isoelectric point: 8.88

Molecular weight: 41.38 kDa

Protein length: 388 aa

Gene length: 1167 bp

Function: nucleotide metabolism

Product: putative hypoxanthine exporter

Essential: no

Synonyms: ydhL

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Nucleotide/ nucleoside transporter
• Metabolism → Nucleotide metabolism → Nucleotide metabolism/ other
• Groups of genes → Membrane proteins

Gene

Coordinates: 625,125 626,291

The protein

Protein family

• major facilitator superfamily (according to UniProt)
• DHA1 family (single member, according to UniProt)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: pbuE
  Description: PubMed

  Regulatory mechanism

  • a-box: termination/antitermination, adenine-controlled, in a-box

  Regulation

  • induced by hypoxanthine and guanine, PurR-independent PubMed
  • induced by adenine (A-box) PubMed

Biological materials

Mutant

• MGNA-C187 (ydhL::erm), available at the NBRP B. subtilis, Japan
• BKE05800 (pbuE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACAAACTCCTTTACTT, downstream forward: _UP4_TCCTTGTAAAAGGAGGATTT
• BKK05800 (pbuE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACAAACTCCTTTACTT, downstream forward: _UP4_TCCTTGTAAAAGGAGGATTT

References

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